期刊
CHEMICAL SCIENCE
卷 11, 期 43, 页码 11729-11739出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0sc04875e
关键词
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资金
- FWO
- European Union [665501, 721613]
- IOF-UGent project F2019/IOF-Concep [TT/188]
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant [721613]
Oligonucleotide-templated reactions are frequently exploited for target detection in biosensors and for the construction of DNA-based materials and probes in nanotechnology. However, the translation of the specifically used template chemistry from solution to surfaces, with the final aim of achieving highly selective high-throughput systems, has been difficult to reach and therefore, poorly explored. Here, we show the first example of a visible light-triggered templated ligation on a surface, employing furan-modified peptide nucleic acids (PNAs). Tailored photo-oxidation of the pro-reactive furan moiety is ensured by the simultaneous introduction of a weak photosensitizer as well as a nucleophilic moiety in the reacting PNA strand. This allows one to ensure a localized production of singlet oxygen for furan activation, which is not affected by probe dilution or reducing conditions. Simple white light irradiation in combination with target-induced proximity between reactive functionalities upon recognition of a short 22mer DNA or RNA sequence that functions as a template, allows sensitive detection of nucleic acid targets in a 96 well plate format.
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