期刊
ADVANCED MATERIALS
卷 32, 期 52, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.202006752
关键词
in vivo imaging; near‐ infrared afterglow; phosphorescence resonance energy transfer; room‐ temperature phosphorescence
类别
资金
- National Natural Science Foundation of China [51973162, 21734007, 51673151]
- Hubei Province [2017CFA002]
- Nanyang Technological University [M4081627]
- Singapore Ministry of Education, Academic Research Fund Tier 1 [2019-T1-002-045, RG125/19]
- Singapore Ministry of Education, Academic Research Fund Tier 2 [MOE2018-T2-2-042]
Afterglow imaging that detects photons after cessation of optical excitation avoids tissue autofluorescence and thus possesses higher sensitivity than traditional fluorescence imaging. Purely organic molecules with room-temperature phosphorescence (RTP) have emerged as a new library of benign afterglow agents. However, most RTP luminogens only emit visible light with shallow tissue penetration, constraining their in vivo applications. This study presents an organic RTP nanoprobe (mTPA-N) with emission in the NIR range for in vivo afterglow imaging. Such a probe is composed of RTP molecule (mTPA) as the phosphorescent generator and an NIR-fluorescent dye as the energy acceptor to enable room-temperature phosphorescence resonance energy transfer (RT-PRET), ultimately resulting in redshifted phosphorescent emission at 780 nm. Because of the elimination of background noise and redshifted afterglow luminescence in a biologically transparent window, mTPA-N permits imaging of lymph nodes in living mice with a high signal-to-noise ratio. This study thus opens up a universal approach to develop organic RTP luminogens into NIR afterglow imaging agents via construction of RT-PRET.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据