期刊
ACS MATERIALS LETTERS
卷 2, 期 11, 页码 1475-1483出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsmaterialslett.0c00428
关键词
-
资金
- National Science Foundation [CMMI-1636136]
- National Institute on Drug Abuse [DA045550]
- NIH Director's Early Independence Award
- National Institute of Dental and Craniofacial Research through the NIH Common Fund [DP5OD028181]
- Alex's Lemonade Stand Foundation for Childhood Cancer Research
- Hyundai Hope on Wheels Foundation for Pediatric Cancer Research
- Tower Cancer Research Foundation
- St. Baldrick's Foundation [SBF586729]
- Office of the Director
Local heating using pulsed-laser-induced photothermal effects on plasmonic nanostructured substrates can be used for intracellular delivery applications. However, the fabrication of plasmonic nanostructured interfaces is hampered by complex nanomanufacturing schemes. Here, we demonstrate the fabrication of large-area plasmonic gold (Au) nanodisk arrays that enable photothermal intracellular delivery of biomolecular cargo at high efficiency. The Au nanodisks (350 nm in diameter) were fabricated using chemical lift-off lithography (CLL). Nanosecond laser pulses were used to excite the plasmonic nanostructures, thereby generating transient pores at the outer membranes of targeted cells that enable the delivery of biomolecules via diffusion. Delivery efficiencies of >98% were achieved using the cell impermeable dye calcein (0.6 kDa) as a model payload, while maintaining cell viabilities at >98%. The highly efficient intracellular delivery approach demonstrated in this work will facilitate translational studies targeting molecular screening and drug testing that bridge laboratory and clinical investigations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据