期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2020.590848
关键词
DNA repair; non-B-DNA; quadruplex; AP endonuclease; nucleotide recognition
资金
- Russian Science Foundation [18-14-00135]
- Russian Ministry of Higher Education and Science [AAAA-A17-117020210022-4]
- Russian Science Foundation [18-14-00135] Funding Source: Russian Science Foundation
The primary role of apurinic/apyrimidinic (AP) endonuclease APE1 in human cells is the cleavage of the sugar phosphate backbone 5 ' to an AP site in DNA to produce a single-strand break with a 5 '-deoxyribose phosphate and 3 '-hydroxyl end groups. APE1 can also recognize and incise some damaged or modified nucleotides and possesses some minor activities: 3 '-5 ' exonuclease, 3 '-phosphodiesterase, 3 '-phosphatase, and RNase H. A molecular explanation for the discrimination of structurally different substrates by the single active site of the enzyme remains elusive. Here, we report a mechanism of target nucleotide recognition by APE1 as revealed by the results of an analysis of the APE1 process involving damaged DNA and native RNA substrates with non-canonical structures. The mechanism responsible for substrate specificity proved to be directly related to the ability of a target nucleotide to get into the active site of APE1 in response to an enzyme-induced DNA distortion.
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