期刊
FRONTIERS IN MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.597794
关键词
avian reovirus; cholesterol; lipid rafts; Mβ CD; virus replication
类别
资金
- National Natural Science Foundation of China [31902250]
- High-end Talent Support Program of Yangzhou University
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- earmarked fund for Modern Agro-industry Technology Research System [CARS-40-K16]
Cholesterol is an essential component of lipid rafts in cellular plasma membranes. Although lipid rafts have been reported to have several functions in multiple stages of the life cycles of many different enveloped viruses, the mechanisms by which non-enveloped viruses, which lack outer lipid membranes, infect host cells remain unclear. In this study, to investigate the dependence of non-enveloped avian reovirus (ARV) infection on the integrity of cholesterol-rich membrane rafts, methyl-beta-cyclodextrin (M beta CD) was used to deplete cellular membrane cholesterol at the ARV attachment, entry, and post-entry stages. Treatment with M beta CD significantly inhibited ARV replication at both the entry and post-entry stages in a dose-dependent manner, but M beta CD had a statistically insignificant effect when it was added at the attachment stage. Moreover, M beta CD treatment markedly reduced syncytium formation, which occurs at a relatively late stage of the ARV life cycle and is involved in cell-cell transmission and release. Furthermore, the addition of exogenous cholesterol reversed the effects mentioned above. Colocalization data also showed that the ARV proteins sigma C, mu NS, and p10 prefer to localize to cholesterol-rich lipid raft regions during ARV infection. Altogether, these results suggest that cellular cholesterol in lipid rafts plays a critical role in ARV replication.
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