期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 21, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/ijms21218313
关键词
hepatic steatosis; histone demethylase Jumonji domain-containing protein 2B; histone methylation; ligand-activated liver X receptor α lipogenesis
资金
- 2020 Post-Doc. Development Program of Pusan National University
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2019R1A2C1090093]
- National Research Foundation of Korea [2019R1A2C1090093] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ligand-activated liver X receptor alpha (LXR alpha) upregulates the expression of hepatic lipogenic genes, which leads to triglyceride (TG) accumulation, resulting in nonalcoholic fatty liver disease (NAFLD). Thus, LXR alpha regulation may provide a novel therapeutic target against NAFLD. However, histone methylation-mediated epigenetic regulation involved in LXR alpha-dependent lipogenesis is poorly understood. In this study, we investigated the functional role of the histone demethylase Jumonji domain-containing protein 2B (JMJD2B) in LXR alpha-dependent lipogenesis. JMJD2B expression level was upregulated in HepG2 cells treated with LXR alpha agonist T0901317 or palmitate and the liver of mice administered with T0901317 or fed a high-fat diet. Knockdown of JMJD2B using siRNA abrogated T0901317-induced LXR alpha-dependent lipogenic gene expression and lowered intracellular TG accumulation. Conversely, overexpression of JMJD2B in HepG2 cells upregulated the expression of LXR alpha-dependent lipogenic genes, in line with increased intracellular TG levels. JMJD2B overexpression or T0901317 treatment induced the recruitment of JMJD2B and LXR alpha to LXR response elements (LXRE) in the promoter region of LXR alpha-target gene and reduced the enrichment of H3K9me2 and H3K9me3 in the vicinity of the LXRE. Furthermore, JMJD2B enhanced T0901317 or LXR alpha-induced transcriptional activities of reporters containing LXRE. A co-immunoprecipitation assay revealed that JMJD2B interacted with activated LXR alpha. Moreover, overexpression of JMJD2B in mice resulted in upregulation of hepatic LXR alpha-dependent lipogenic genes, consistent with development of hepatic steatosis. Taken together, these results indicate that JMJD2B plays a role in LXR alpha-mediated lipogenesis via removing the repressive histone marks, H3K9me2 and H3K9me3, at LXRE, which might contribute to hepatic steatosis.
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