期刊
FRONTIERS IN NEUROANATOMY
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fnana.2020.582218
关键词
immunofluorescence; paraffin sections; antibodies; microscopy; imaging
资金
- German Research Foundation (DFG) [SFB665, SFB1315]
- Charite-Universitatsmedizin Berlin
- German Academic Exchange Service (DAAD)
Immunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and the websites of commercial antibody producers. This can result in a time-consuming and costly methodical work to establish simple antibody staining. We here summarize in a stepwise manner an easy-to-follow immunofluorescence staining protocol with an improved specific fluorescent signal and a reduced background and non-specific binding signal. This will help scientists to save time, effort, and antibody costs during the application of such a valuable technique.
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