期刊
TOXICON
卷 187, 期 -, 页码 255-261出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2020.09.005
关键词
Paralytic shellfish poisoning toxin; Saxitoxin; Seafood; Surface plasmon resonance; Attenuated internal reflection ellipsometry
资金
- Scientific and Technological Research Council of Turkey (TUBITAK) [119Z191]
In this study, we proposed label-free saxitoxin (STX) sensor using STX specific aptamer in combination with spectroscopic ellipsometry (SE) and attenuated internal reflection (AIR) spectroscopic ellipsometry method which is operated under surface plasmon resonance (SPR) conditions. Besides the other surface plasmon resonance-based applications, AIR-SE applications have unique advantages in terms of sensitivity and it was used herein for real-time detection of STX in real samples. Another method, SE, was also used and compared with AIR-SE. Analytical performances were satisfactory with low detection limits and a wide detection range. Limit of detection was 0.01 ng/mL for AIR-SE and 0.11 ng/mL for SE. Both proposed sensors were operable in 0.01 nM-1000 nM STX range. These methods were also used for the accurate, selective, and sensitive detection of STX from fish and shrimp samples.
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