期刊
SCIENCE ADVANCES
卷 6, 期 47, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abc1204
关键词
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资金
- National Science and Technology Major Project Key New Drug Creation and Manufacturing Program [2018ZX09201002-006]
- National Natural Science Foundation of China [81770039, 81570028, 81490533, 81400018]
- National Key RD Plan [2016YFC1304104]
- Zhongshan Hospital Clinical Research Foundation [2016ZSLC05, 2019ZSGG15]
- Shanghai Municipal Key Clinical Specialty [shslczdzk02201]
- Shanghai Top-Priority Clinical Key Disciplines Construction Project [2017ZZ02013]
MicroRNAs (miRNAs), mRNA, and proteins in/on extracellular vesicles (EVs) represent potential cancer biomarkers. Concurrent detection of multiple biomarkers at a single-EV level would greatly improve prognosis and/or diagnosis and understanding of EV phenotypes, biogenesis, and functions. Here, we introduced a High-throughput Nano-bio Chip Integrated System for Liquid Biopsy (HNCIB) system for simultaneous detection of proteins and mRNA/miRNA in a single EV. Validated through systematic control experiments, HNCIB showed high reliability, sensitivity, and specificity. In a panel of 34 patients with lung adenocarcinoma (LUAD) and 35 healthy donors, HNCIB detected an up-regulated expression of programmed death-ligand 1 mRNA and protein and miR-21 in EVs derived from patients with LUAD compared to those from healthy donors. HNCIB has low sample requirement (similar to 90 mu l), fast assay time (similar to 6 hours), and high throughput (up to 384 samples per assay) and would have great potential in the study of EVs and their clinical applications.
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