期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 191, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2020.113583
关键词
Salvia miltiorrhiza; Polysaccharides; saccharides mapping; HPSEC; Chemometrics
资金
- National Natural Science Foundation of China [81673389]
- National Key R&D Program of China [2019YFC1711300]
- Science and Technology Development Fund, Macau SAR [0075/2018/A2, 034/2017/A1, 0017/2019/AKP]
- Key Area Research and Development Program of Guangdong Province [2020B1111110006]
- University of Macau [MYRG2018-00083-ICMS/MYRG2019-00128-ICMS/CPG2020-00021-ICMS]
Comparison of Salvia miltiorrhiza polysaccharides (SMPs) from different geographical origins in China (Henan, Hebei, Shandong, Sichuan, Shaanxi) was performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), saccharide mapping based on polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) and combined with principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Based on the results of HPSEC-MALLS/RI, the relative content of SMPs showed a significant difference between different geographical origins, however, the molecular weight of SMPs showed almost no significance. SMPs can be discriminated as five regions after PACE coupled with OPLS-DA models analysis of endo-1,5-alpha-arabinanase hydrolysates. Moreover, all the PACE fingerprint indicated that 1,4-beta-Galp, 1,5-alpha-Araf, 1,4-alpha-D-GalAp and 1,4-beta-D-Glcp linkages existed in SMPs. (C) 2020 Elsevier B.V. All rights reserved.
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