期刊
SCIENTIFIC DATA
卷 7, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41597-020-00719-4
关键词
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资金
- National Key Research and Development Program of China, Stem Cell and Translational Research [2017YFA0106800]
- National Science Fund for Excellent Young Scholars [81722004]
- Canadian Institutes of Health Research [CIHR EP1-120608, EP2-120609]
- EU FP7 High Impact Project BLUEPRINT [HEALTH-F5-2011-282510]
Both poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses. To better access these effects, we carried out both ribosomal RNA-depleted and poly(A)-selected RNA-seq for CD4+ T naive cells isolated from 40 healthy individuals from the Blueprint Project. For these 40 individuals, the genomic and epigenetic data were also available. This dataset offers a unique opportunity to understand how library construction influences differential gene expression, alternative splicing and molecular QTL (quantitative loci) analyses for human primary cells.
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