期刊
RSC ADVANCES
卷 10, 期 69, 页码 42076-42083出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ra09110c
关键词
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资金
- National Natural Science Foundation of China [91753130]
- Excellent Young Scientists Fund of China (Hong Kong and Macau) [21922708]
- Hong Kong Research Grants Council (RGC) Collaborative Research Fund (CRF) [C7029-15G, C7017-18G]
- Areas of Excellence Scheme [AoE/P-705/16, 17121120, 17126618, 17125917]
- RGC Postdoctoral Fellowship
Protein-protein interactions (PPIs) play crucial roles in regulating essentially all cellular processes. Photo-cross-linking represents a powerful method to study PPIs. To fulfil the requirements for the exploration of different PPIs, there is a continuous demand on the development of novel photo-reactive amino acids with diverse structural properties and functionalities. Reported herein is the development of a bifunctional amino acid termed dzANA, which contains a diazirine, for photo-cross-linking, and a terminal alkyne group, for bioorthogonal tagging. Using known PPIs between histone posttranslational modifications (PTMs) and their binding partners as models, we demonstrate that the dzANA-harbouring peptide-based photoaffinity probes could efficiently and selectively capture the weak and transient PPIs mediated by histone modifications. Our study indicates the potential of dzANA to identify and characterize unknown PPIs.
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