期刊
BIOMOLECULES
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/biom10111532
关键词
magnoflorine; natural products; isoquinoline alkaloids; anti-cancer activity; Berberis cretica; Berberidaceae; counter-current chromatography; CPC; HPLC-MS
资金
- Medical University of Lublin, Poland [MNmb48]
- Medical University of Lublin [DS 440]
- European Union (ERDF)
- Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation [5002803]
Magnoflorine (MGN) is a quaternary aporphine alkaloid that exhibits numerous therapeutic properties, including neuropsychopharmacological, anti-anxiety, immunomodulatory, anti-inflammatory, antioxidant, or antifungal activities. The aim of the present study was an investigation of the influence of MGN on viability, proliferation, induction of apoptosis, and cell cycle arrest in NCI-H1299 lung, MDA-MB-468 breast, T98G glioma, and TE671 rhabdomyosarcoma cancer cells. MGN was isolated from the roots of Berberis cretica L. by counter-current partition chromatography (CPC). Cell viability and proliferation assessments were performed by means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and 5-bromo-2MODIFIER LETTER PRIME-deoxyuridine (BrDU) assays, respectively. The induction of apoptosis and cell cycle progression was measured using fluorescence-activated cell sorting analysis. MGN in high doses inhibits proliferation, induces apoptosis, and inhibits cell cycle in S/G2 phases in a dose-dependent manner. MGN seems to be a promising anti-cancer compound in therapy of some types of lung, breast, glioma, and rhabdomyosarcoma cancers, for which current standard therapies are limited or have severe strong side effects.
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