期刊
BIOSENSORS-BASEL
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/bios10110175
关键词
respiratory syncytial virus; cyclic voltammetry; electrochemical impedance spectroscopy; sensor; gold electrode; glassy carbon
资金
- First TEAM program of the Foundation for Polish Science [POIR.04.04.00-00-1644/18]
- National Science Centre (Poland) [2018/29/B/NZ2/00668]
This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 x 10(5) PFU/mL to 1.5x10(7) PFU/mL, more than 1.0 x 10(5) PFU/mL to 1.0 x 10(7) PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 x 10(3) PFU/mL, three orders of magnitude lower than 2.85 x 10(6) PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.
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