4.6 Article

Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies

期刊

MICROORGANISMS
卷 8, 期 11, 页码 -

出版社

MDPI
DOI: 10.3390/microorganisms8111659

关键词

porcine intestinal epithelial cells; wakame; pigs; Ligilactobacillus salivarius; adhesion; immunomodulation; genomics

资金

  1. project of NARO Bio-oriented Technology Research Advancement Institution (research program on the development of innovative technology) [01002A]
  2. Japan Society for the Promotion of Science (JSPS) [19H00965]
  3. Association for Research on Lactic Acid Bacteria
  4. ANPCyT-FONCyT [PICT-2016-0410]
  5. Tohoku University Research Program Frontier Research in Duo (FRiD)
  6. Ministry of Education, Culture, Science, Sports, and Technology (MEXT) of Japan [16H06429, 16K21723, 16H06435]
  7. JSPS
  8. JSPS (Postdoctoral Fellowship for Foreign Researchers) [18F18081]
  9. Tohoku University Global Hagi Scholarship
  10. Japan Racing Association
  11. [19K22300]
  12. Grants-in-Aid for Scientific Research [18F18081] Funding Source: KAKEN

向作者/读者索取更多资源

In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different immune and adhesion phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.

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