期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 163, 期 -, 页码 776-787出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.07.069
关键词
Glucoglucuronan; Laminarin; Anti-lung cancer activity
资金
- Zhejiang Provincial Natural Science Foundation of China [LY19D060006]
- National Natural Science Foundation of China [41906095, 41506165, 81771520]
- China Scholarship Council
- National Institutes of Health [DK111958, CA231074]
Laminarin (LA), a storage glucan, was purified from the brown alga Sargassum thunbergii. After specific oxidation using the stable nitroxyl radical, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO), together with NaBr and NaClO, glucoglucuronan (LAO) was obtained. Compositional analysis of LAO showed a molar ratio of glucuronic acid (GlcA) to glucose (Glc) of 12.7: 1. Nuclear magnetic resonance (NMR) and mass spectroscopy (MS) showed LAO to have a backbone of (1 -> 3)-linked beta-D-GlcpA interspersed with (1 -> 3, 1 -> 6)-linked beta-D-Glcp, that was terminated with beta-D-GlcpA. LAO inhibited human lung cancer A549 cell proliferation in vitro. IC50 values at 12 h and 24 h were 2.70 mg/mL and 2.85 mg/mL, respectively. Western blotting showed that TSC2 was up-regulated at an LAO concentration of 3.80 mg/mL. FAK, PI3K, P-AKT and mTOR were down-regulated, indicating LAO inhibited cancer cell proliferation through the FAK/PI3K/AKT/mTOR pathway. Surface plasmon resonance (SPR) studies revealed that LAO showed an IC50 of 0.07 mg/mL inhibiting the binding of heparin to fibroblast growth factor 1 (FGF1) LAO inhibition of heparin binding to FGF2 fluctuated between 15% and 28%, suggesting that LAO inhibits A549 cell proliferation by selectively interacting with FGF1. (C) 2020 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据