期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 163, 期 -, 页码 959-969出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.07.072
关键词
Fucan; Silver nanoparticles, AgNP; Wound healing
资金
- Applied Molecular Biosciences Unit-UCIBIO - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia e do Ensino Superior, FCT/MCTES [UID/Multi/04378/2019]
- Materials Research Center, CENIMAT/i3N iNOVA4 - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia e do Ensino Superior, FCT/MCTES [UID/CTM/50025/2013]
- Health Research Unit - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia e do Ensino Superior, FCT/MCTES [LISBOA-01-0145-FEDER-007344]
- ERDF, under the PT2020 Partnership Agreement [POCI-01-0145-FEDER-007728]
- FCT/MCTES [SFRH/BD/131947/2017, SFRH/BD/115173/2016]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/131947/2017] Funding Source: FCT
This study demonstrates the potential of a high molecular weight fucose-containing polysaccharide secreted by the bacterium Enterobacter A47, named FucoPol, and its silver nanocomposite as potential bioactive materials for wound dressings applications. A green, simple, light-assisted method was used for the synthesis of silver nanoparticles (AgNP) using FucoPol, as stabilizing and reducing agent. The synthesized nanoparticles were spherical, and the main population had a particle size in number ranging between 13 and 30 nm for percentiles 50 and 90, respectively. FucoPol, as well as the functionalized material, besides having no cytotoxicity towards human skin keratinocytes and mouse fibroblasts, also promoted in vitro keratinocytes migration. These observations not only show the safety of FucoPol and FucoPol/AgNP biocomposite, but also their wound healing ability. Moreover, the biocomposite had a strong antimicrobial activity against Staphylococcus aureus ATCC 6538 and Klebsiella pneumoniae CECT 8453, two representative strains of known skin commensal pathogens. These findings demonstrate for the first time the potential of FucoPol for the development of wound healing formulations. Additionally, the FucoPol/AgNP biocomposite might find use in antimicrobial biomaterials, including antibacterial wound healing formulations, which further strengthens the establishment of FucoPol as a bioactive biopolymer. (C) 2020 Elsevier B.V. All rights reserved.
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