期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 163, 期 -, 页码 1037-1043出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.07.052
关键词
Chitinases; CsChiE; Hydrolysis; GlcNAc; Chitobiose; Bioconversion
资金
- Department of Science and Technology (DST), Government of India, Funds for Infrastructure in Science and Technology (FIST), Level II
- University Grants Commission
- UGC
- Dr. D. S. Kothari postdoctoral fellowship scheme [BSR/BL/16-17/0344]
- DST-INSPIRE-Faculty award [IFA16-LSPA 40]
- DST, Govt of India
Enzymatic deconstruction of chitin to chitobiose is of significant interest in view of its various biological applications. Here we report detailed insights into the chitin degradation by chitinase-E from a chitinolytic bacterium Chitiniphilus shinanonensis (CsChiE). CsChiE was optimally active at 50 degrees C in 50 mM sodium phosphate pH-7.0. It showed a k(cat) and overall catalytic efficiency (K-cat/K-m) as 3.9 x 10(3) s(-1) and 0.6 x 10(3) s(-1) mg(-1) mL, respectively, on colloidal chitin (CC). CsChiE efficiently hydrolyzed crystalline polymers like alpha-, beta- and CC and released chitobiose as the predominant product (11.3 mM on CC). Further, CsChiE displayed substantial activity towards the unmilled crab shell chitin waste (chitin-flakes) and generated chitobiose. Activity studies on chitooligosaccharides revealed that CsChiE produced chitobiose as the major product. Our results indicate that the multi-modular CsChiE is a non-processive exo-chitinase which is more suitable to generate chitobiose from a variety of chitinous substrates including unprocessed chitin-flakes. (C) 2020 Elsevier B.V. All rights reserved.
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