期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 163, 期 -, 页码 1393-1402出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.07.229
关键词
Polysaccharides; Ziziphus jujuba Mill var. spinosa seeds; Spinosin; Absorption; Anti-inflammatory
资金
- Tianjin First, Second and Third Industry Integration Development Technology Demonstration Project [17ZXYENC00190]
In this study, the effect of Ziziphus jujuba Mill var. spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated. After continuously fed with ZSS polysaccharides 100mg/kg.d(-1) for 28 consecutive days, the C57BL/6mice absorbed spinosin at an obvious lower level compared with the control group. The expression levels of P-gp, MRP2 and Occludin in the colon were significantly increased. ZSS polysaccharides significantly regulated the composition of the gutmicrobiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia. Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells. However, the gut microbiota culture supernatant showed no obvious biological activity in this regard. Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice. This immune regulation was related to the NF-kappa B and MAPK pathways in RAW264.7 cells. (C) 2020 Elsevier B.V. All rights reserved.
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