期刊
STAR PROTOCOLS
卷 1, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2020.100169
关键词
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资金
- National Key Research and Development Program of China [2018YFC1200100]
- National Science and Technology Major Project [2018ZX10301403]
- Ministry of Science and Technology [2020YFC0841400, 2020B111108001, 2018B020207013]
- National Institutes of Health USA (NIH) [P01 AI060699, RO1 AI129269]
- Center for Gene Therapy for Cystic Fibrosis (NIH) [P30 DK-54759]
- Cystic Fibrosis Foundation
- Roy J. Carver Charitable Trust
Common laboratory mice such as BALB/c and C57BL/6 mice are not permissive to SARS-CoV2 infection. Sensitization of laboratory mice with Adenovirus express-ing human ACE2 (Ad5-hACE2) provides a rapid model for testing viral interven-tion in vivo. Despite the lack of lethal outcome, Ad5-hACE2-sensitized mice show 20% weight loss on average upon viral challenge with infectious virus being de-tected at the site of sensitization. This protocol describes the sensitization and subsequent infection of common laboratory mice for use in testing anti-viral in-terventions.For complete details on the use and execution of this protocol, please refer to Sun et al. (2020).
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