期刊
STAR PROTOCOLS
卷 1, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2020.100148
关键词
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资金
- Helmholtz Zentrum Munchen - German ResearchCenter for Environmental Health - Federal Ministry of Education and Research (BMBF)
- State of Bavaria
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [SFB 1371, 395357507]
- Technical University of Munich
Targeted sequencing of 16S rRNA genes enables the analysis of microbiomes. Here, we describe a protocol for the collection, storage, and preparation of fecal samples. We describe how we cluster similar sequences and assign bacterial taxonomies. Using diversity analysis and machine learning, we can extract dis-ease-associated features. We also describe a circadian analysis to identify the presence or absence of rhythms in taxonomies. Differences in rhythmicity between cohorts can contribute to determining disease-associated bacterial signatures. For complete details on the use and execution of this protocol, please refer to Reitmeier et al. (2020).
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