期刊
STAR PROTOCOLS
卷 1, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2020.100118
关键词
-
资金
- German Research Foundation [KL 973/12-1, LI 3716/1-1]
- Else Kroner-Fresenius-Stiftung (EKFS)
Studying brown and brite adipose tissue requires precise and reliable quantification of cellular thermogenesis. This protocol describes the isolation of primary murine pre-adipocytes, differentiation into thermogenic brown and brite adipocytes, and subsequent oxygen consumption analysis. Commonly applied procedures only mea-sure basal and maximal proton leak-linked oxygen consumption but not explicitly un-coupling protein 1 (UCP1)-dependent respiration. Meaningful oxygen consumption analyses require (1) the activation of UCP1, (2) control over intracellular free-fatty -acid levels, and (3) inhibition of ATP-consuming futile cycles.For complete details on the use and execution of this protocol, please refer to Li et al. (2014, 2017, 2018) and Schweizer et al. (2018).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据