期刊
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
卷 82, 期 -, 页码 19-25出版社
INDIAN PHARMACEUTICAL ASSOC
DOI: 10.36468/pharmaceutical-sciences.spl.76
关键词
Metabolic engineering; Kennedy pathway; fatty acids; particle bombardment; Acrodactylum Tricornutum
资金
- development special funds of Shenzhen strategic emerging industries and future industries [201708021308]
- Shenzhen science and technology application demonstration project [KJYY20180201180253571]
- Shenzhen Grant Plan for Science and Technology [JCYJ20170818092708470, JCYJ20170302144706218]
- Natural Science Foundation of Guangdong Province [2018A030310446]
Li: LPAAT gene in Phaeodactylum tricornutum enhances fatty acid accumulation To provide a potential method for improving microalgal lipid production, an over expression vector that expresses lysophosphatidic acid acyltransferase gene in Phaeodactylum tricornutum was constructed. Total fatty acid production incresed significantly in over-expression transformants and the length of fatty acids ranged from C14 to C22. The response to both lysophosphatidic acid acyltransferase over- and under-expression over the growth phase was also characterized. This study indicated a potential method to increase fatty acids accumulation in microalgae by employing a metabolic engineering approach.
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