期刊
GENE REPORTS
卷 21, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.genrep.2020.100900
关键词
Antimicrobial peptide; Pichia pastoris; Melittin; Gene expression; Drug resistance
资金
- Deputy of Research, Mashhad University of Medical Sciences [970784]
With the emergence of antibiotic resistance, researchers have recently focused on antimicrobial peptides as potential drug candidates. In this study, Melittin, one of the most effective antimicrobial peptides of honey bee venom was produced by the expression system of Pichia pastoris GS115. Melittin was cloned into pPIC9 vector, transformed, and integrated into the P. pastoris genome. The highest level of expression was observed in BMM minimal medium without protein, pH 6, 2% methanol, and 48 h. Then, the recombinant Melittin was tested on some gram-positive and gram-negative bacteria to investigate the antimicrobial effects and showed widespectrum activity. The production of Melittin by this method costs very low and paves the way for further studies and also therapeutic usages.
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