期刊
NEUROSCIENCE LETTERS
卷 739, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2020.135399
关键词
Exosomes; Human placenta-derived mesenchymal stem cells; Angiogenesis; Spinal cord injury
资金
- Hunan Provincial Nature Science Youth Fund [2019JJ50936, 2019JJ50959]
- National Natural Science Foundation of China [81874004, 81262174]
- Science and Technology Commission of Hunan Province of China [2017SK2061]
- Science Foundation of Xiangya Hospital for Young Scholar [2017Q1]
Background: Spinal cord injury (SCI) is a serious central nervous system condition with no effective clinal treatment. Recently, transplantation of bone marrow mesenchymal stem cells (MSCs) derived exosomes has been proposed as a potential treatment for SCI. However, whether exosomes have similar functions as transplanted human placenta-derived MSCs(hPMSCs) has remained unclear. Methods: The hPMSCs-derived exosomes (hPMSCs-Exos) were extracted using a sequential centrifugation approach. Then, the effects of hPMSCs-Exos on angiogenesis were analysis both in vitro and in vivo. In addition, the sensory and locomotor functions of mice after SCI were also analyzed. Results: The administration of hPMSCs-Exos promote the tube formation and migration of human umbilical vein endothelial cell (HUVECs). Furthermore, vessel numbers, vessel volume fraction and vessel connectivity in spinal cords significantly increased after exosomes were intrathecally injected in the SCI model. In addition, the locomotor and sensory function, also significantly improved in the exosome treatment group. Conclusions: The results of the present study demonstrated that hPMSCs-Exos have proangiogenic effects on endothelial cells and enhanced angiogenesis in SCI model. Thus, this treatment strategy demonstrates great potential for the treatment of SCI.
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