期刊
BREEDING SCIENCE
卷 70, 期 5, 页码 540-546出版社
JAPANESE SOC BREEDING
DOI: 10.1270/jsbbs.20020
关键词
carrot; EMS; mutagenesis; mutant
资金
- Key Program of Key Research the Development Plan of Shanxi Province [201703D211001-04-01]
- National Natural Science Foundation of China [3160110604]
- Key Research and Development Plan of Shanxi Province [201903D221063]
- Science and Technology Innovation Project of Shanxi Agricultural University [2016ZZ02]
Carrot is among the top-ten most economically important vegetable crops in the world. However, the gene pool of cultivated carrot is narrow and the collection and preservation of carrot germplasm are very limited in China. In this study, seeds of carrot inbred line 17005 were treated by ethyl methyl sulfone (EMS) to construct a mutant library. The conditions of EMS mutagenesis on inbred line 17005 were optimized, including treatment of seeds at 0.5% EMS for 6 h We obtained a number of mutant lines showing inheritable morphological mutations in cotyledon. growth point. leaf taproot in the vegetative phase and mutations in bolting time, primary stem height and color, secondary branch, flower and seed in the reproductive phase through M-2 and M-3. The F-2 segregating population from the cross of yellow taproot and purple-red epidermis taproot with wild type showed that these two mutants were controlled by single recessive gene respectively. The inheritable mutants can not only enrich the carrot germplasm, but also provide resources for the function research of carrot gene.
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