期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 8, 期 46, 页码 10593-10601出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0tb00779j
关键词
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资金
- Major R&D Program of the National Ministry of Science and Technology [2018YFB1105504]
- National Natural Science Foundation [11672002, 31771322, 31571235, 31571236]
- Beijing Municipal Science and Technology Commission Science and Technology Nova Cross Project [2018019]
- Ministry of Education Innovation Program of China [IRT16R01, IRT1201]
- Key Laboratory of Trauma and Nerve Regeneration, theMinistry of Education (2018)
- National Center for Trauma Medicine
- Chinese National Ministry of Science and Technology 973 Project [2014CB542201]
Graphene oxide is currently used in peripheral nerve engineering but has certain limitations, such as cytotoxicity and lack of electrical conductivity, both of which are crucial in regulating nerve-associated cell behaviors. In this work, we engineered reduced graphene oxide-GelMA-PCL nanofiber nerve guidance conduits via electrospinning. rGO incorporated into the GelMA/PCL matrix significantly enhanced the electrical conductivity and biocompatibility of the hybrid materials. In addition, hybrid nanofibers with low concentrations of rGO (0.25 and 0.5 wt%) could significantly improve the proliferation of Schwann cells (RSC96). More importantly, rGO/GelMA/PCL hybrid nanofibers could activate the epithelial-mesenchymal transition (EMT)-related gene expression of Schwann cells (RSC96). From the in vivo study, it was observed that rGO/GelMA/PCL nerve guidance conduits could promote both sensory/motor nerve regeneration and functional recovery in rats. Our composite strategy of combining rGO within a biocompatible nanofiber scaffold is simple but effective in improving tissue engineering outcomes. The rGO/GelMA/PCL hybrid nanofibers have great potential in peripheral nerve tissue engineering. They will also provide an experimental basis for the development of further electrical stimulation in peripheral nerve regeneration.
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