期刊
ACS APPLIED BIO MATERIALS
卷 3, 期 8, 页码 4902-4911出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.0c00421
关键词
cell-penetrating peptides; cyclodextrins; intracellular delivery; proteins; liposomes; supramolecular chemistry
资金
- MEXT/JSPS KAKENHI [15H02569, 16K13092, 17H02208, 18KK0156, 19K22260, 19K22972, 20H02871]
- MEXT-Supported Program for the Strategic Research Foundation at Private Universities (2015-2019)
- Takeda Science Foundation
- NOVARTIS Foundation (Japan) for the Promotion of Science
- Suntory Foundation for Life Sciences
- JGC-S Scholarship Foundation
- Grants-in-Aid for Scientific Research [20H02871, 19K22260, 19K22972, 17H02208, 18KK0156, 15H02569, 16K13092] Funding Source: KAKEN
Herein, we demonstrate a convenient technique for the intracellular delivery of proteins and liposomes based on supramolecular host-guest chemistry. First, we prepared the R8-CDOH carrier molecule, which is a ss-cyclodextrin derivative bearing an octaarginine (R8) chain, as a cellpenetrating peptide, at the primary hydroxyl group. The surface amino groups of proteins (GFP, ss-gal, and IgG) were then partly modified with adamantane (Ad) tags using 1-Ad-carboxylic acid N-hydroxysuccinimide ester (Ad-NHS). These Ad-tagged proteins were effectively delivered into HeLa cells though supramolecular host-guest interactions with R8-CDOH. A 100 nm sized liposome bearing Ad-tags on its surface was also delivered into these cells by the action of R8-CDOH. The present method does not require any genetic manipulation, and only easy chemical modification processes are used to facilitate intracellular delivery; therefore, we believe that the present method is applicable to a variety of bioengineering processes, such as protein-based therapeutics, cellular reprogramming, and genome-editing, among others.
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