Anti-Trichomonas vaginalis Effect of Methanolic Extracts of Sambucus nigra in Comparison with Metronidazole

Background: Trichomoniasis is the most common non-viral sexually transmitted disease caused by a flagellated protozoan living in the genitourinary tract, which infects both men and women. Metronidazole is the treatment of choice for trichomoniasis. Researchers are seeking an alternative to metronidazole because of its inevitable side effects and toxicity. Objectives: This study aimed to evaluate the effect of the methanolic extract of Sambucus nigra against Trichomonas vaginalis in vitro. Methods: Plants were collected from different areas of Mazandaran Province, northern Iran. Fruits were separated, shade-dried, milled, and their methanolic extract was prepared in concentrations of 100, 200, 400, and 800 mu g/mL. Parasites were obtained from patients referring to different health centers of Mazandaran province, cultured in Dorset medium, and incubated at 37 degrees C. The effects were evaluated and compared to a control group. The data were analyzed by SPSS 18 using the ANOVA test. Results: The exposure time and concentration of the extracts had a direct effect on anti-parasitic activity so that increasing extract concentration and incubation time heightened the anti-trichomoniasis effects. The concentrations of 400 and 800 mu g/ml of the plant had 100% efficacy after 72 and 48 hours, respectively. Conclusions: It can be concluded from our results that the methanolic extract of S. nigra has a remarkable ability to destroy T. vaginalis and it can be considered an effective drug against T. vaginalis with further studies in human and animal models.