期刊
ACS APPLIED ELECTRONIC MATERIALS
卷 2, 期 11, 页码 3610-3617出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsaelm.0c00677
关键词
insect odorant receptor; lipid nanodisc; liposome; Orco; biosensor; GFET; electronic nose
资金
- Royal Society Rutherford Postdoctoral Scholarship [RFT-PAF1501-PD]
- Marsden Fund [PAF1503]
- MacDiarmid Institute for Advanced Materials and Nanotechnology
- Kiwi Innovation Network (KiwiNET) Inaugural Emerging Innovator Award
- Marsden Fund grant from Royal Society of New Zealand
Graphene field effect transistor (GFET) biosensors have been used to evaluate insect odorant receptor (OR) display formats and the effect of the presence of the Orco subunit on in vitro sensing using OR subunits. The biosensors were fabricated by immobilizing the OR nanodiscs and OR +/- Orco liposomes onto GFET devices. Two ORs from Drosophila melanogaster (Dme-IOR10a and DmelOR22a) were used in this study. We demonstrated that both DmelORIOa and DmelOR22a in nanodiscs and OR +/- Orco liposomes showed a selective electrical response to their respective positive ligands, methyl salicylate and methyl hexanoate. The OR nanodisc sensors produced the strongest sensing signal with a limit of detection of 1 fm. We also found that the upper detection limit was increased, and the sensitivity of the Orco coexpressed liposome sensors was enhanced by approximately an order of magnitude when compared to the OR liposome sensors.
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