期刊
METHODSX
卷 7, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mex.2020.100809
关键词
CoroNa-Green AM dye; Na+ fluorescence intensity; Plant growth; Propidium iodide; Salt stress; Z-stack
资金
- Department of Biotechnology
Domestication and cultivation of tree species, such as Pongamia pinnata is quite important because of its biofuel properties. Seedlings grown in modified hydroponic culture were morphologically similar to that of soil grown seedlings. Further, seedlings were allowed to grow without root limitation. Comparatively, our modified hydroponic growth system can be performed with minimal resources. Prior incubated root segments with CoroNa-Green AM dye retained maximum amount of dye when compared to CoroNa-Green AM dye incubated sections. Our modified protocol provides quantitative analysis of 2D and 3D imaging process at cellular and sub-cellular level. Our protocol is customized to study individual plant behavior. Additionally, it is customized for growing tap rooted trees species hydroponically. Changing the nutrient solution with regular intervals provides continuous supply of nutrients to the plants. Prior incubation of root segments with Na+ probe (CoroNa-Green AM) provides better resolution in imaging process. Additionally, both 2D and 3D imaging provides a means to acquire and analyze entirety of the sample. (C) 2020 The Authors. Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据