期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 47, 页码 13815-13823出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c04434
关键词
Adelphocoris lineolatus; odorant receptor AlinORS9; floral scents; two-electrode voltage clamp recordings; methyl salicylate; behavioral response
资金
- National Natural Science Foundation of China [31672038, 31772176, 31972338]
- National Key Research and Development Program of China [2019YFD0300100]
- Key Research and Development Program of Guangdong Province [2019B020217003-05]
- Rural Revitalization Strategy Project of Guangdong Province [2019KJ122]
Plant volatiles such as floral scent compounds play a crucial role in mediating insect host locating, mate search, and oviposition sites selection. The alfalfa plant bug, Adelphocoris lineolatus (Goeze), is a seriously polyphagous herbivore of alfalfa and cotton that has an obvious preference for flowering host plants. In this study, we focused on the role of an odorant receptor AlinOR59 in the perception of plant volatiles in A. lineolatus. In situ hybridization showed that AlinOR59 was coexpressed with the coreceptor AlinORco in the ORNs cell located in the long curved sensilla trichodea on antennae of both genders. The Xenopus oocytes expression coupled with two-electrode voltage clamp recordings demonstrated that AlinOR59 responded to 15 plant volatiles. In electroantennogram assays, all of the above 15 compounds could excite electrophysiological responses in the antennae of adult bugs. Furthermore, an important floral scent compound, methyl salicylate, was utilized to evaluate the behavioral responses of A. lineolatus. It was found that adult bugs of both genders were significantly attracted to methyl salicylate. Taken together, our findings suggest that AlinORS9 plays a crucial role in the perception of floral scents in A. lineolatus and could be used as a potential target to design novel olfactory regulators for the management of bugs.
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