期刊
HUMAN IMMUNOLOGY
卷 78, 期 2, 页码 103-112出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2016.11.006
关键词
NK cells; Cytotoxicity; NCR; Reference range; Subset; Receptor
类别
资金
- Basic Science Research Program through National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2015R1D1A1A09058740]
- Korea Research Institute of Bioscience and Biotechnology [KGM4941612]
- Chonnam National University Hospital Research Institute of Clinical Medicine [CRI13047-21]
- National Research Council of Science & Technology (NST), Republic of Korea [KGM4941612] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2015R1D1A1A09058740] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Background: The purpose of this study was to identify CD56(bright) and CD56(dim) natural killer (NM) cell subsets and analyze their receptors expression in a healthy Korean population, and to determine whether receptor expression correlates with age, sex, and cytotoxicity. Materials and methods: We performed multicolor flow cytometry assays to analyze the expression of various NM cell receptors (CD16, NKG2A, NKG2C, NKG2D, CD57, DNAM-1, CD8a, CD62L, NKp30, and NKp46) on both CD3(-)/CD56(dim) and CD3(-)/CD56(bright) NK cells in whole-blood samples from 122 healthy donors. The expression of these receptors was compared according to age (<30 years, n = 22, 30-60 years, n = 73 and >60 years, n = 27) and gender (male, n = 61, female, n = 61). NK cell cytotoxicity assays were performed with peripheral blood mononuclear cells (PBMCs) from 18 individuals. The results were compared to the expression levels of NKp30 and NKp46 receptors. Results: A normal reference range for NK cell receptor expression in two NK cell subsets was established. NKp46 and NKG2D expression gradually decreased with age (p < 0.01 and p < 0.05, respectively) whereas NK cell proportion and numbers, frequencies of CD56(dim) cells, and CD57 expression increased with age (p < 0.01 in all cases). Men showed greater NM cell proportion and numbers, frequencies of CD56(dim) cells, and CD57 expression compared to those of women (p < 0.05 and p < 0.001; p < 0.01 and p < 0.01, respectively). Notably, the expression of NKp46 was negatively correlated with NM cell frequency (r = -0.42, p < 0.001). Furthermore, NM cell cytotoxicity was found to positively correlate with NCR expression (p = 0.02), but not NK cell proportion (p = 0.80). Conclusion: We have established a profile of NM cell surface receptors for a Korean population, and revealed that age and gender have an effect on the expression of NK cell receptors in the population. Our data might explain why neither NM cell numbers nor proportions correlate with NM cell cytotoxicity. (C) 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
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