Potential of plant growth promoting Rhizobacteria to overcome the exposure of pesticide in Trigonella foenum - graecum (fenugreek leaves)

Agrochemicals are generally used as additional supplements to the soil for improving soil fertility throughout the world. In this present study was carried out to characterize the pesticide tolerance plant growth promoting rhizobacteria (PGPR) were isolated from fenugreek leaves. Seven bacterial isolates were obtained namely FOW1 to FOW7 and characterized on their colony morphology, size and colour. Among the seven, FOW1 and FOW7 isolates were found to grown well in nutrient medium containing 10-20% concentration of pesticide. All the seven isolates were produced indole-3-acetic acid in the range of 56-97 mu g/mL and only two isolates (FOW1 and FOW7) displayed the phosphate solubilising activity in Pikovskaya agar, whereas six isolates were showed positive for production of siderophore. Out of seven isolates, two exhibited in vitro plant growth promotion activities and indicated that these isolates can be exploited as biofertilizers and microbial inoculants for crops as they enhanced plant growth via diverse mechanisms and offered an attractive strategy to replace synthetic fertilizers and pesticides. The morphological features such as germinating ability, shoot length, root length were enhanced in the presence of PGPR and was maximum at 1%.