期刊
BMC RESEARCH NOTES
卷 13, 期 1, 页码 -出版社
SPRINGERNATURE
DOI: 10.1186/s13104-020-04971-0
关键词
T-cells; GSK-3; Motility; Cell contacts
资金
- Wellcome Trust [092627/Z/10/Z]
- Canadian Institutes of Health Foundation [159912]
- Wellcome Trust ISSF Award [204825/Z/16/Z]
- Wellcome Trust [092627/Z/10/Z] Funding Source: Wellcome Trust
ObjectiveThe threonine/serine kinase glycogen synthase kinase 3 (GSK-3) targets multiple substrates in T-cells, regulating the expression of Tbet and PD-1 on T-cells. However, it has been unclear whether GSK-3 can affect the motility of T-cells and their interactions with antigen presenting cells.ResultsHere, we show that GSK-3 controls T-cell motility and interactions with other cells. Inhibition of GSK-3, using structurally distinct inhibitors, reduced T-cell motility in terms of distance and displacement. While SB415286 reduced the number of cell-cell contacts, the dwell times of cells that established contacts with other cells did not differ for T-cells treated with SB415286. Further, the increase in cytolytic T-cell (CTL) function in killing tumor targets was not affected by the inhibition of motility. This data shows that the inhibition of GSK-3 has differential effects on T-cell motility and CTL function where the negative effects on cell-cell interactions is overridden by the increased cytolytic potential of CTLs.
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