Microfluidics for the rapid detection of Staphylococcus aureus using antibody-coated microspheres

Staphylococcus aureusis a common foodborne pathogenic microorganism which can cause food poisoning and it is pathogenic to both humans and animals. Therefore, rapid detection ofS. aureusinfection is of great significance. In this study, a microfluidic platform was introduced to detectS. aureusby fluorescence labeling method and a self-made microfluidic chip, which has immune spheres were used to study the effect of capturingS. aureus. Through this experiment, we found that the platform can be used for microbial culture, andS. aureusantibody coated on the diameter of 50 similar to 90 mu m microspheres for detection. On the premise of optimizing the sample flow rate and detection time, the bacterial detection was quantitatively monitored. Results showed that our platform can detectS. aureusat injection rate of 5 mu L center dot min(-1)reacted for 4 min and the detection limit of bacteria is 1.5 x 10(1)CFU/mu L. However, the detection time of traditional method is 24 hs to 72 hs, and the operation is complex and cumbersome. These findings indicated that the microfluidic chip in this study is portable, sensitive, and accurate, laying a good foundation for further research on the application of rapid bacterial detection platform.