期刊
HEARING RESEARCH
卷 344, 期 -, 页码 284-294出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.heares.2016.12.006
关键词
Auditory brainstem; Cochlear nucleus; Endbulb of Held synapses; Ultrastructure; Postsynaptic density; Auditory experience
资金
- National Institutes of Deafness and Others Communication Disorders [R01DC013048]
- Pennsylvania Lions Hearing Research Foundation
- Department of Otolaryngology of the University of Pittsburgh
AMPA glutamate receptor complexes with fast kinetics conferred by subunits like GIuA3 and GluA4 are essential for temporal precision of synaptic transmission. The specific role of GluA3 in auditory processing and experience related changes in the auditory brainstem remain unknown. We investigated the role of the GIuA3 in auditory processing by using wild type (WT) and GluA3 knockout (GluA3-KO) mice. We recorded auditory brainstem responses (ABR) to assess auditory function and used electron microscopy to evaluate the ultrastructure of the auditory nerve synapse on bushy cells (AN-BC synapse). Since labeling for GIuA3 subunit increases on auditory nerve synapses within the cochlear nucleus in response to transient sound reduction, we investigated the role of GIuA3 in experience-dependent changes in auditory processing. We induced transient sound reduction by plugging one ear and evaluated ABR threshold and peak amplitude recovery for up to 60 days after ear plug removal in WT and GluA3-K0 mice. We found that the deletion of GIuA3 leads to impaired auditory signaling that is reflected in decreased ABR peak amplitudes, an increased latency of peak 2, early onset hearing loss and reduced numbers and sizes of postsynaptic densities (PSDs) of AN-BC synapses. Additionally, the lack of GluA3 hampers ABR threshold recovery after transient ear plugging. We conclude that GluA3 is required for normal auditory signaling, normal ultrastructure of AN-BC synapses in the cochlear nucleus and normal experience-dependent changes in auditory processing after transient sound reduction. (C) 2016 Elsevier B.V. All rights reserved.
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