期刊
GENETICS
卷 208, 期 2, 页码 605-622出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.117.300268
关键词
Drosophila; ecdysone; heterochromatin; steroid hormone biosynthesis; transcription; zinc finger
资金
- Japan Society for the Promotion of Science
- Precursory Research for Embryonic Science and Technology of the Japan Science and Technology Agency
- program of the Joint Usage/Research Center for Developmental Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University
- Natural Sciences and Engineering Research Council (NSERC) Canada
- NSERC [RGPIN 341543]
- Canadian Institutes of Health Research [MOP 93761]
Ecdysteroids are steroid hormones that control many aspects of development and physiology. During larval development, ecdysone is synthesized in an endocrine organ called the prothoracic gland through a series of ecdysteroidogenic enzymes encoded by the Halloween genes. The expression of the Halloween genes is highly restricted and dynamic, indicating that their spatiotemporal regulation is mediated by their tight transcriptional control. In this study, we report that three zinc finger-associated domain (ZAD)-C2H2 zinc finger transcription factorsSeance (Sean), Ouija board (Ouib), and Molting defective (Mld)cooperatively control ecdysone biosynthesis in the fruit fly Drosophila melanogaster. Sean and Ouib act in cooperation with Mld to positively regulate the transcription of neverland and spookier, respectively, two Halloween genes. Remarkably, loss-of-function mutations in sean, ouib, or mld can be rescued by the expression of neverland, spookier, or both, respectively. These results suggest that the three transcription factors have distinct roles in coordinating the expression of just two genes in Drosophila. Given that neverland and spookier are located in constitutive heterochromatin, Sean, Ouib, and Mld represent the first example of a transcription factor subset that regulates genes located in constitutive heterochromatin.
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