期刊
GENETICS
卷 207, 期 2, 页码 729-740出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.117.300221
关键词
vector genetics; hybrid incompatibility; gene flow; Y chromosome; malaria
资金
- European Union's Seventh Framework Programme (FP7) Marie Curie Actions cofund (Project I-Move) [267232]
- Foundation for the National Institutes of Health through the Vector-Based Control of Transmission: Discovery Research (VCTR) program of the Grand Challenges in Global Health initiative of the Bill & Melinda Gates Foundation
- Rita Levi Montalcini award from the Ministry Education, University and Research (MIUR) [79 04.02.2014]
- European Research Council under the European Union's Seventh Framework Programme ERC [335724]
- MRC Career Development Award [G1100339]
- Wellcome Trust [098051]
- European Research Council (ERC) [335724] Funding Source: European Research Council (ERC)
- Medical Research Council [G1100339] Funding Source: researchfish
- MRC [G1100339] Funding Source: UKRI
Y chromosome function, structure and evolution is poorly understood in many species, including the Anopheles genus of mosquitoes-an emerging model system for studying speciation that also represents the major vectors of malaria. While the Anopheline Y had previously been implicated in male mating behavior, recent data from the Anopheles gambiae complex suggests that, apart from the putative primary sex-determiner, no other genes are conserved on the Y. Studying the functional basis of the evolutionary divergence of the Y chromosome in the gambiae complex is complicated by complete F1 male hybrid sterility. Here, we used an F1 x F0 crossing scheme to overcome a severe bottleneck of male hybrid incompatibilities that enabled us to experimentally purify a genetically labeled A. gambiae Y chromosome in an A. arabiensis background. Whole genome sequencing ( WGS) confirmed that the A. gambiae Y retained its original sequence content in the A. arabiensis genomic background. In contrast to comparable experiments in Drosophila, we find that the presence of a heterospecific Y chromosome has no significant effect on the expression of A. arabiensis genes, and transcriptional differences can be explained almost exclusively as a direct consequence of transcripts arising from sequence elements present on the A. gambiae Y chromosome itself. We find that Y hybrids show no obvious fertility defects, and no substantial reduction in male competitiveness. Our results demonstrate that, despite their radically different structure, Y chromosomes of these two species of the gambiae complex that diverged an estimated 1.85 MYA function interchangeably, thus indicating that the Y chromosome does not harbor loci contributing to hybrid incompatibility. Therefore, Y chromosome gene flow between members of the gambiae complex is possible even at their current level of divergence. Importantly, this also suggests that malaria control interventions based on sex-distorting Y drive would be transferable, whether intentionally or contingent, between the major malaria vector species.
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