期刊
GENES TO CELLS
卷 22, 期 2, 页码 189-202出版社
WILEY
DOI: 10.1111/gtc.12467
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资金
- AMED
- CREST
- Ministry of Education, Culture, Sports, Science and Technology of Japan
The Activin/Nodal/TGF-beta signaling pathway plays a major role in maintaining mouse epiblast stem cells (EpiSCs). The EpiSC-maintaining medium, which contains Activin A and bFGF, induces differentiation of mouse embryonic stem cells (ESCs) to EpiSCs. Here, we show that Activin A also has an ability to efficiently propagate ESCs without differentiation to EpiSCs when combined with a MEK inhibitor PD0325901. ESCs cultured in Activin+PD retained high-level expression of naive pluripotency-related transcription factors. Genomewide analysis showed that the gene expression profile of ESCs cultured in Activin+PD resembles that of ESCs cultured in 2i. ESCs cultured in Activin+PD also showed features common to the naive pluripotency of ESCs, including the preferential usage of the Oct4 distal enhancer and the self-renewal response to Wnt pathway activation. Our finding shows a role of Activin/Nodal/TGF-beta signaling in stabilizing self-renewal gene regulatory networks in ESCs.
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