Arthrobacter sulfonylureivorans sp. nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil

A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117(T), was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil. The optimal temperature and pH for the growth of strain LAM7117(T) were 35 degrees C and 7.5, respectively. Strain LAM7117(T) could grow in the presence of NaCl with concentration up to 9% (w/v). Strain LAM7117(T) formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522(T) (97.7%). The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117(T) and A. crystallopoietes JCM 2522(T) were 21.4 and 77.4%, respectively. The genomic DNA G + C content was 65.9 mol%. The major cellular fatty acids were anteiso-C-15:0, iso-C-16:0 and anteiso-C-17:0. The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid. The major polar lipids present in strain LAM7117(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid. The predominant menaquinones of strain LAM7117(T) were MK-8 and MK-9. Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117(T) should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp. nov. is proposed. The type strain is LAM7117(T) (= JCM 32824(T) = CGMCC 1.16681(T)).

Automated summary

Strain LAM7117(T) is a Gram-positive rod-shaped bacterium that can grow at 35 degrees Celsius and pH 7.5, withstanding NaCl concentrations up to 9%. It belongs to a distinct phylogenetic subclade within the genus Arthrobacter, showing highest similarity with A. crystallopoietes JCM 2522(T) (97.7%). The genomic analyses suggest it should be classified as a novel species, named Arthrobacter sulfonylureivorans sp. nov.