期刊
GENES & DEVELOPMENT
卷 31, 期 18, 页码 1870-1879出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.301093.117
关键词
transcription termination; RNA polymerase II; XRN2; Caenorhabditis elegans
资金
- National Institutes of Health Office of Research Infrastructure Programs [P40 OD010440]
- Swiss National Science Foundation [SNF 31003A_143313]
- Novartis Research Foundation through Friedrich Miescher Institute for Biomedical Research Foundation
- Swiss National Science Foundation (SNF) [31003A_143313] Funding Source: Swiss National Science Foundation (SNF)
Transcription termination determines the ends of transcriptional units and thereby ensures the integrity of the transcriptome and faithful gene regulation. Studies in yeast and human cells have identified the exoribonuclease XRN2 as a key termination factor for protein-coding genes. Here we performed a genome-wide investigation of RNA polymerase II (Pol II) transcription termination in XRN2-deficient Caenorhabditis elegans and observed two distinct modes of termination. Although a subset of genes requires XRN2, termination of other genes appears both independent of, and refractory to, XRN2. XRN2 independence is not merely a consequence of failure to recruit XRN2, since XRN2 is present on-and promotes Pol II accumulation near the polyadenylation sites of-both gene classes. Unexpectedly, promoters instruct the choice of termination mode, but XRN2-independent termination additionally requires a compatible region downstream from the 3' end cleavage site. Hence, different termination mechanisms may work with different configurations of Pol II complexes dictated by promoters.
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