期刊
GENES & DEVELOPMENT
卷 31, 期 4, 页码 347-352出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.291500.116
关键词
WT1; FLASH; 3 ' UTR; hybrids; RNA secondary structures; developmental pathways
资金
- Medical Research Council [MR/N020405/1]
- BBSRC [BB/L020416/1] Funding Source: UKRI
- MRC [MR/M010341/1, MR/N020405/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L020416/1] Funding Source: researchfish
- Kidney Research UK [RP23/2009] Funding Source: researchfish
- Medical Research Council [MR/N020405/1, MR/M010341/1] Funding Source: researchfish
Wilms' tumor 1 (WT1) is essential for the development and homeostasis of multiple mesodermal tissues. Despite evidence for post-transcriptional roles, no endogenous WT1 target RNAs exist. Using RNA immunoprecipitation and UV cross-linking, we show that WT1 binds preferentially to 3' untranslated regions (UTRs) of developmental targets. These target mRNAs are down-regulated upon WT1 depletion in cell culture and developing kidney mesenchyme. Wt1 deletion leads to rapid turnover of specific mRNAs. WT1 regulates reporter gene expression through interaction with 3' UTR-binding sites. Combining experimental and computational analyses, we propose that WT1 influences key developmental and disease processes in part through regulating mRNA turnover.
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