Influence of cationic surfactants and inorganic salts on the enzyme kinetic activity of Mucor javanicus lipase

In this article, the enzyme kinetic activity of Mucor javanicus lipase (MJL) has investigated via hydrolysis of p-nitrophenyl acetate (PNPA) in different pH environment, cationic surfactants, and inorganic salts. The influence of hydrophilicity has been studied in the presence of cationic cetyltrimethylammonium bromide (CTAB), cetyltriphenylphosphonium bromide (CTPB), and cetyldimethylethanolammonium bromide (CDMEAB) surfactants. The head group dimensions of surfactants show noteworthy contributions to enhance the catalytic activity of MJL, that is the CDMEAB surfactant, which is more efficient when compared with CTPB and CTAB. The increase in hydrophobicity of alkyltrimethylammonium bromide and alkyltriphenylphosphonium bromide in series dodecyl (C-12)( ) tetradecyl (C-14)( ) cetyl (C-16) leads to a marked decrease in the observed enzymatic superactivity, but an opposite trend appears in case of alkyldimethylammonium bromide. The activity of MJL for the hydrolysis of PNPA was higher in the presence of sodium chloride and potassium chloride anion due to specific interactions between the lipase surface and anions of the Hofmeister effect.

Automated summary

This study investigated the enzyme kinetic activity of MJL under different conditions, showing significant influences of surfactants and salts on its catalytic activity. Hydrophilic surfactants enhance the catalytic activity, while sodium chloride and potassium chloride contribute to an increase in activity.