期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 243, 期 -, 页码 96-119出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2016.11.001
关键词
Crustacea; Decapoda; Neurohormone; Neuropeptide; Transcriptomics; X-organ-sinus gland system
资金
- National Science Foundation [NSF] [ABI-1062432]
- NSF [IOS-1353023, IOS-1354567, OCE-1459235]
- National Institutes of Health [5P20RR016463-12, 8P20GM103423-12]
- Cades Foundation of Honolulu, Hawaii
- University of Hawaii at Manoa's Undergraduate Research Opportunities Program
- American Physiological Society Undergraduate Research Fellowship Program
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1353023] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1354567] Funding Source: National Science Foundation
In silico transcriptome mining is a powerful tool for crustacean peptidome prediction. Using homology based BLAST searches and a simple bioinformatics workflow, large peptidomes have recently been predicted for a variety of crustaceans, including the lobster, Homarus americanus. Interestingly, no in silico studies have been conducted on the eyestalk ganglia (lamina ganglionaris, medulla externa, medulla interna and medulla terminalis) of the lobster, although the eyestalk is the location of a major neuroendocrine complex, i.e., the X-organ-sinus gland system. Here, an H. americanus eyestalk ganglia-specific transcriptome was produced using the de novo assembler Trinity. This transcriptome was generated from 130,973,220 Illumina reads and consists of 147,542 unique contigs. Eighty-nine neuropeptide-encoding transcripts were identified from this dataset, allowing for the deduction of 62 distinct pre/preprohormones. Two hundred sixty-two neuropeptides were predicted from this set of precursors; the peptides include members of the adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin B, allatostatin C, bursicon alpha, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone (CHH), CHH precursor-related peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, elevenin, FMRFamide-like peptide, glycoprotein hormone alpha 2, glycoprotein hormone beta 5, GSEFLamide, intocin, leucokinin, molt-inhibiting hormone, myosuppressin, neuroparsin, neuropeptide F, orcokinin, orcomyotropin, pigment dispersing hormone, proctolin, pyrokinin, red pigment concentrating hormone, RYamide, short neuropeptide F, SIFamide, sulfakinin, tachykinin-related peptide and trissin families. The predicted peptides expand the H. americanus eyestalk ganglia neuropeptidome approximately 7-fold, and include 78 peptides new to the lobster. The transcriptome and predicted neuropeptidome described here provide new resources for investigating peptidergic signaling within/ from the lobster eyestalk ganglia. (C) 2016 Elsevier Inc. All rights reserved.
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