Amycolatopsis acididurans sp. nov., isolated from peat swamp forest soil in Thailand

A polyphasic approach was used to describe strain K13G38(T), a novel actinomycete isolated from peat swamp forest soil collected from Surat Thani Province, Thailand. The 16S rRNA gene phylogenetic analysis indicated that the strain belonged to the genus Amycolatopsis and showed the highest sequence similarities to both Amycolatopsis acidiphila JCM 30562(T) and Amycolatopsis bartoniae DSM 45807(T) (96.8% sequence similarity). Furthermore, strain K13G38(T), which formed extensively branched substrate and aerial mycelia, exhibited chemotaxonomical characteristics of the genus Amycolatopsis which included phospholipid pattern type II and cell-wall chemotype IV. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, two unidentified phospholipids, and an unidentified aminolipid. MK-9(H-4) was a predominant menaquinone of the organism. The major cellular fatty acids were iso-C-16:0, anteiso-C-17:0, and C-16:0. The genomic DNA size of strain K13G38(T) was 8.5 Mbp with 69.5 mol% G+C content. On the basis of phenotypic characteristics, overall genomic relatedness index and phylogenetic distinctiveness, strain K13G38(T) represents a novel species of the genus Amycolatopsis, for which the name A. acididurans sp. nov. is proposed. The type strain is K13G38(T) (=TBRC 12507(T) = NBRC 114553(T)).

Automated summary

A novel actinomycete strain K13G38(T) isolated from peat swamp forest soil in Thailand was phylogenetically identified as a new species of the genus Amycolatopsis, named A. acididurans. The strain displayed typical chemotaxonomic characteristics of the genus and exhibited distinctive features at both chemical and genomic levels compared to known species in the same genus.