期刊
ANNALS OF BIOMEDICAL ENGINEERING
卷 49, 期 3, 页码 1058-1068出版社
SPRINGER
DOI: 10.1007/s10439-020-02649-z
关键词
Imaging; Multi-scale testing; Buffer solution; TEM
资金
- National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases [AR070966]
- National Institutes of Health, National Institute of General Medical Sciences [P20GM103446]
The study compared the effects of two different bathing solutions on tendon multiscale mechanics and microstructure, showing that physiological PBS led to decreased tendon modulus and microstructural changes, while SPEG solution provided some protection.
One of the most common bath solutions used in musculoskeletal mechanical testing is phosphate buffered saline (PBS). In tendon, swelling induced by physiological PBS results in decreased tendon modulus and induces microstructural changes. It is critical to evaluate the multiscale mechanical behavior of tendon under swelling to interpret prior work and provide information to design future studies. We compared the effects of physiological PBS and 8% polyethylene glycol and saline bathing solutions on tendon multiscale tendon mechanics and damage as well as microstructure with TEM in order to understand the effect of swelling on tendon. At the tissue level, tendons in PBS had a lower modulus than SPEG samples. PBS samples also showed an increased amount of non-recoverable sliding, which is an analog for microscale damage. SPEG had a higher microscale to tissue-scale strain ratio, showing the fibrils experienced less strain attenuation. From the TEM data, we showed the fibril spacing of SPEG samples was more similar to fresh control than PBS. We concluded that swelling alters multiscale mechanics and damage in addition to tendon microstructure. Future mechanical testing should consider using SPEG as a bath solution with an osmotic pressure which preserves fresh tissue water content.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据