期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 413, 期 2, 页码 519-531出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-03022-8
关键词
Extracellular substances; Lactobacillus plantarum; Extraction and quantification; Physicochemical characterization; LC-MS/MS; Proteins identification
资金
- Regional Council of Bourgogne Franche-Comte
- Fonds Europeen de Developpement Regional (FEDER) [CRB 2016-9201AAO048S01632]
- Ministere de l Enseignement superieur, de la Recherche et de l Innovation, France
Sugar-enriched media are used to produce extracellular substances by Lactobacillus plantarum, with distinct physicochemical patterns observed at different growing stages. A higher production of proteinaceous materials compared to polysaccharides is highlighted in the study.
Sugar-enriched media are used to produce extracellular substances (ECS) by Lactobacillus plantarum WCSF1, with a focus on growing stages and carbon source substrates. Combination of size exclusion chromatography and ATR-FTIR spectroscopy provides physicochemical patterns of bulk ECS produced along culture growing time. Secreted biopolymers present polydisperse and high molecular weight distributions, with significant amounts of carbohydrates and proteins. Results, supported by a multivariate statistical analysis, enable to differentiate the macromolecular content of bacterial ECS along the growing stages regardless of the growing media, highlighting a higher production of proteinaceous materials compared to polysaccharides. At the end of the exponential phase, common exoproteins were present in all the tested sugar-enriched media such as transglycosylases between 20 and 35 kDa, a muropeptidase at 36.9 kDa and a cell wall hydrolase. Additionally, L. plantarum WCFS1 secretes ECS with a greater diversity of proteins, when growing in the sucrose-enriched media.
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