期刊
EPIGENETICS
卷 16, 期 10, 页码 1102-1122出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15592294.2020.1841872
关键词
Toxicoepigenetics; DNA methylation; Developmental origins of health and disease (DOHaD); surrogate tissue; sex differences; environmental epigenomics
资金
- National Institute of Environmental Health Sciences [P01 ES022844/RD83543601, ES026697, ES028802, T32 ES007062, P30 ES017885]
This study explores the sex-specific effects of perinatal exposure to Pb on DNA methylation in mice, finding thousands of differentially methylated cytosines, including imprinted loci, in both liver and blood tissues. Furthermore, a subset of Pb-altered genes exhibit basal sex differences in gene expression in the mouse liver, providing potential molecular targets for Pb-induced metabolic diseases and informing the design of more robust human environmental epigenomics studies.
Early developmental environment can influence long-term health through reprogramming of the epigenome. Human environmental epigenetics studies rely on surrogate tissues, such as blood, to assess the effects of environment on disease-relevant but inaccessible target tissues. However, the extent to which environment-induced epigenetic changes are conserved between these tissues is unclear. A better understanding of this conservation is imperative for effective design and interpretation of human environmental epigenetics studies. The Toxicant Exposures and Responses by Genomic and Epigenomic Regulators of Transcription (TaRGET II) consortium was established by the National Institute of Environmental Health Sciences to address the utility of surrogate tissues as proxies for toxicant-induced epigenetic changes in target tissues. We and others have recently reported that perinatal exposure to lead (Pb) is associated with adverse metabolic outcomes. Here, we investigated the sex-specific effects of perinatal exposure to a human environmentally relevant level of Pb on DNA methylation in paired liver and blood samples from adult mice using enhanced reduced-representation bisulphite sequencing. Although Pb exposure ceased at 3 weeks of age, we observed thousands of sex-specific differentially methylated cytosines in the blood and liver of Pb-exposed animals at 5 months of age, including 44 genomically imprinted loci. We observed significant tissue overlap in the genes mapping to differentially methylated cytosines. A small but significant subset of Pb-altered genes exhibit basal sex differences in gene expression in the mouse liver. Collectively, these data identify potential molecular targets for Pb-induced metabolic diseases, and inform the design of more robust human environmental epigenomics studies.
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