期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 99, 期 2, 页码 699-728出版社
WILEY
DOI: 10.1002/jnr.24754
关键词
auditory development; auditory neuron diversity; axon initial segment; HCN channels; prestin; RRID; AB_90725; RRID; AB_2039906; RRID; AB_2302038; RRID; AB_2313584; RRID; AB_2313726; RRID; AB_2336419; RRID; AB_2336420; RRID; AB_2336790; RRID; AB_2340477; RRID; AB_2340452; RRID; AB_2340593; RRID; AB_2341028; RRID; AB_2617143; RRID; AB_2756625; RRID; AB_2756742; RRID; SCR_002865; RRID; SCR_013652; RRID; SCR_014823; sound coding; spiral ganglion neurons; voltage gated
资金
- MedEl Innsbruck
- Austrian Science Fund
Neuronal diversity in the cochlea is largely influenced by ion channels, particularly the HCN channels which play a crucial role in auditory function. Studying the expression levels and spatial distribution of HCN subunits across different mammalian species can provide insights into age-related hearing deterioration mechanisms and potentially offer solutions for hearing impairments.
Neuronal diversity in the cochlea is largely determined by ion channels. Among voltage-gated channels, hyperpolarization-activated cyclic nucleotide-gated (HCN) channels open with hyperpolarization and depolarize the cell until the resting membrane potential. The functions for hearing are not well elucidated and knowledge about localization is controversial. We created a detailed map of subcellular location and co-expression of all four HCN subunits across different mammalian species including CBA/J, C57Bl/6N, Ly5.1 mice, guinea pigs, cats, and human subjects. We correlated age-related hearing deterioration in CBA/J and C57Bl/6N with expression levels of HCN1, -2, and -4 in individual auditory neurons from the same cohort. Spatiotemporal expression during murine postnatal development exposed HCN2 and HCN4 involvement in a critical phase of hair cell innervation. The huge diversity of subunit composition, but lack of relevant heteromeric pairing along the perisomatic membrane and axon initial segments, highlighted an active role for auditory neurons. Neuron clusters were found to be the hot spots of HCN1, -2, and -4 immunostaining. HCN channels were also located in afferent and efferent fibers of the sensory epithelium. Age-related changes on HCN subtype expression were not uniform among mice and could not be directly correlated with audiometric data. The oldest mice groups revealed HCN channel up- or downregulation, depending on the mouse strain. The unexpected involvement of HCN channels in outer hair cell function where HCN3 overlaps prestin location emphasized the importance for auditory function. A better understanding may open up new possibilities to tune neuronal responses evoked through electrical stimulation by cochlear implants.
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